› 4xs6

X-ray diffraction
3.35Å resolution

Salmonella typhimurium AhpC W81F mutant

Released:
DOI: 10.2210/pdb4xs6/pdb
PDB entry 4xs6 coloured by chain, front view.
PDB entry 4xs6 coloured by chain, side view.
PDB entry 4xs6 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Experimentally Dissecting the Origins of Peroxiredoxin Catalysis.
Nelson KJ, Perkins A, Van Swearingen AED, Hartman S, Brereton AE, Parsonage D, Salsbury FR, Karplus PA, Poole LB
Antioxid Redox Signal 28 521-536 (2018)
PMID: 28375740

Function and Biology Details

Reaction catalysed: 
NADH + ROOH + H(+) = NAD(+) + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkyl hydroperoxide reductase C Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 186 amino acids
Theoretical weight: 20.6 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A251 (Residues: 2-187; Coverage: 100%)
Gene names: STM0608, ahpC
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand K 4 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2221
Unit cell:
a: 125.47Å b: 169.92Å c: 134.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.279
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Glutathione-Related Enzymes and Proteins: A Review.
Vašková et al. (2023)
2 more

1 mention without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)