› 4xp1

X-ray diffraction
2.89Å resolution

X-ray structure of Drosophila dopamine transporter bound to neurotransmitter dopamine

Released:
DOI: 10.2210/pdb4xp1/pdb
PDB entry 4xp1 coloured by chain, front view.
PDB entry 4xp1 coloured by chain, side view.
PDB entry 4xp1 coloured by chain, top view.
Source organisms:
Primary publication:
Neurotransmitter and psychostimulant recognition by the dopamine transporter.
Wang KH, Penmatsa A, Gouaux E
Nature 521 322-7 (2015)
PMID: 25970245

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212463 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Sodium-dependent dopamine transporter Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 60.02 KDa
Source organism: Drosophila melanogaster
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7K4Y6 (Residues: 25-600; Coverage: 85%)
Gene names: CG8380, DAT, fmn
Sequence domains: Sodium:neurotransmitter symporter family
Antibody fragment light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.31 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
antibody fragment heavy chain Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
8 bound ligands:
Ligand NA 3 x NA
Ligand CL 1 x CL
Ligand NAG 1 x NAG
Ligand P4G 1 x P4G
Ligand LDP 1 x LDP
Ligand EDO 1 x EDO
Ligand Y01 1 x Y01
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 96.797Å b: 140.181Å c: 166.195Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.256
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

37 review citations

Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.
Fairweather et al. (2021)
36 more

36 mentions without citation

Monoamine transporters: insights from molecular dynamics simulations.
Grouleff et al. (2015)
35 more