› 4xnf

X-ray diffraction
1.68Å resolution

Tailspike protein double mutant D339A/E372Q of E. coli bacteriophage HK620

Released:
DOI: 10.2210/pdb4xnf/pdb
PDB entry 4xnf coloured by chain, front view.
PDB entry 4xnf coloured by chain, side view.
PDB entry 4xnf coloured by chain, top view.
Source organism: Enterobacteria phage HK620
Entry authors: Gohlke U, Broeker NK, Heinemann U, Seckler R, Barbirz S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophage P22 tailspike N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 599 amino acids
Theoretical weight: 64.55 KDa
Source organism: Enterobacteria phage HK620
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9AYY6 (Residues: 112-710; Coverage: 84%)
Gene name: 9
Sequence domains: Phage spike trimer
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TRS 1 x TRS
Ligand FMT 5 x FMT
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P321
Unit cell:
a: 74.222Å b: 74.222Å c: 174.337Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.154 0.196
Expression system: Escherichia coli BL21

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