› 4x5t

X-ray diffraction
3.5Å resolution

alpha 1 glycine receptor transmembrane structure fused to the extracellular domain of GLIC

Released:
DOI: 10.2210/pdb4x5t/pdb
PDB entry 4x5t coloured by chain, front view.
PDB entry 4x5t coloured by chain, side view.
PDB entry 4x5t coloured by chain, top view.
Source organisms:
Primary publication:
Allosteric and hyperekplexic mutant phenotypes investigated on an α1 glycine receptor transmembrane structure.
Moraga-Cid G, Sauguet L, Huon C, Malherbe L, Girard-Blanc C, Petres S, Murail S, Taly A, Baaden M, Delarue M, Corringer PJ
Proc Natl Acad Sci U S A 112 2865-70 (2015)
PMID: 25730860

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149954 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine receptor subunit alpha-1; Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 330 amino acids
Theoretical weight: 37.88 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 44-160, 165-235; Coverage: 60%)
  • Canonical: P23415 (Residues: 173-176, 248-338, 418-446; Coverage: 29%)
Gene names: GLRA1, glr4197, glvI
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NI 6 x NI
Ligand CL 6 x CL
Ligand ACT 5 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 118.894Å b: 132.304Å c: 190.517Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.254 0.27
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Emerging Molecular Mechanisms of Signal Transduction in Pentameric Ligand-Gated Ion Channels.
Nemecz et al. (2016)
3 more

5 mentions without citation

Structure and Pharmacologic Modulation of Inhibitory Glycine Receptors.
Burgos et al. (2016)
4 more