› 4x2s

X-ray diffraction
4.21Å resolution

Crystal structure of 276S/M395R-GltPh in inward-facing conformation

Released:
DOI: 10.2210/pdb4x2s/pdb
PDB entry 4x2s coloured by chain, front view.
PDB entry 4x2s coloured by chain, side view.
PDB entry 4x2s coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Transport domain unlocking sets the uptake rate of an aspartate transporter.
Akyuz N, Georgieva ER, Zhou Z, Stolzenberg S, Cuendet MA, Khelashvili G, Altman RB, Terry DS, Freed JH, Weinstein H, Boudker O, Blanchard SC
Nature 518 68-73 (2015)
PMID: 25652997

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate transporter homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 422 amino acids
Theoretical weight: 44.57 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59010 (Residues: 1-419; Coverage: 99%)
Gene name: PH1295
Sequence domains: Sodium:dicarboxylate symporter family

Ligands and Environments

2 bound ligands:
Ligand ASP 3 x ASP
Ligand NA 6 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6522
Unit cell:
a: 121.651Å b: 121.651Å c: 578.699Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.28 0.278 0.314
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
21 more

10 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
9 more