› 4wol

X-ray diffraction
1.77Å resolution

Crystal Structure of the DAP12 transmembrane domain in lipidic cubic phase

Released:
DOI: 10.2210/pdb4wol/pdb
PDB entry 4wol coloured by chain, front view.
PDB entry 4wol coloured by chain, side view.
PDB entry 4wol coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Transmembrane Complexes of DAP12 Crystallized in Lipid Membranes Provide Insights into Control of Oligomerization in Immunoreceptor Assembly.
Knoblich K, Park S, Lutfi M, van 't Hag L, Conn CE, Seabrook SA, Newman J, Czabotar PE, Im W, Call ME, Call MJ
Cell Rep 11 1184-92 (2015)
PMID: 25981043

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TYRO protein tyrosine kinase-binding protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 33 amino acids
Theoretical weight: 3.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43914 (Residues: 35-67; Coverage: 36%)
Gene names: DAP12, KARAP, TYROBP

Ligands and Environments

2 bound ligands:
Ligand K 1 x K
Ligand OLC 4 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 20.775Å b: 35.709Å c: 101.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.183 0.223
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Progress and prospects for structural studies of transmembrane interactions in single-spanning receptors.
Trenker et al. (2016)
11 other articles

1 mention without citation

Transmembrane Complexes of DAP12 Crystallized in Lipid Membranes Provide Insights into Control of Oligomerization in Immunoreceptor Assembly.
Knoblich et al. (2015)