› 4wnp

X-ray diffraction
1.88Å resolution

Structure of ULK1 bound to a potent inhibitor

Released:
DOI: 10.2210/pdb4wnp/pdb
PDB entry 4wnp coloured by chain, front view.
PDB entry 4wnp coloured by chain, side view.
PDB entry 4wnp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human autophagy initiating kinase ULK1 in complex with potent inhibitors.
Lazarus MB, Novotny CJ, Shokat KM
ACS Chem Biol 10 257-61 (2015)
PMID: 25551253

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase ULK1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 287 amino acids
Theoretical weight: 32.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75385 (Residues: 1-283; Coverage: 27%)
Gene names: KIAA0722, ULK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand 3RJ 4 x 3RJ
1 modified residue:
Ligand TPO 4 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P2221
Unit cell:
a: 100.41Å b: 113.8Å c: 100.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.226
Expression system: Escherichia coli

Quick links

Citations

47 review citations

Mammalian Autophagy: How Does It Work?
Bento et al. (2016)
46 more

7 mentions without citation

Structure and function of the ULK1 complex in autophagy.
Lin et al. (2016)
6 more