› 4wie

X-ray diffraction
2.18Å resolution

Crystal structure of apo-PEPCK from Mycobacterium tuberculosis with glycerol

Released:
DOI: 10.2210/pdb4wie/pdb
PDB entry 4wie coloured by chain, front view.
PDB entry 4wie coloured by chain, side view.
PDB entry 4wie coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Ra
Entry authors: Kim HL, Sacchettini JC

Function and Biology Details

Reaction catalysed: 
GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107722 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase [GTP] Chain: A
Molecule details ›
Chain: A
Length: 622 amino acids
Theoretical weight: 69.05 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Escherichia coli
UniProt:
  • Canonical: A5TYT6 (Residues: 1-606; Coverage: 100%)
Gene names: MRA_0219, pckG
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 103.958Å b: 122.564Å c: 64.555Å
α: 90° β: 117° γ: 90°
R-values:
R R work R free
0.141 0.138 0.191
Expression system: Escherichia coli

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