› 4wib

X-ray diffraction
3.2Å resolution

Crystal structure of Magnesium transporter MgtE

Released:
DOI: 10.2210/pdb4wib/pdb
PDB entry 4wib coloured by chain, front view.
PDB entry 4wib coloured by chain, side view.
PDB entry 4wib coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structural basis for ion selectivity revealed by high-resolution crystal structure of Mg2+ channel MgtE.
Takeda H, Hattori M, Nishizawa T, Yamashita K, Shah ST, Caffrey M, Maturana AD, Ishitani R, Nureki O
Nat Commun 5 5374 (2014)
PMID: 25367295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Magnesium transporter MgtE Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 19.25 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SMG8 (Residues: 271-448; Coverage: 40%)
Gene names: TTHA1060, mgtE
Sequence domains: Divalent cation transporter
Structure domains: SLC41 divalent cation transporters, integral membrane domain

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P212121
Unit cell:
a: 64.12Å b: 70.38Å c: 103.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.268
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Metal homeostasis and resistance in bacteria.
Chandrangsu et al. (2017)
3 more

2 mentions without citation

Structural basis for ion selectivity revealed by high-resolution crystal structure of Mg2+ channel MgtE.
Takeda et al. (2014)
1 more