› 4wc4

X-ray diffraction
3.5Å resolution

tRNA-processing enzyme complex 2

Released:
DOI: 10.2210/pdb4wc4/pdb
PDB entry 4wc4 coloured by chain, front view.
PDB entry 4wc4 coloured by chain, side view.
PDB entry 4wc4 coloured by chain, top view.
Source organisms:
Primary publication:
Measurement of Acceptor-TΨC Helix Length of tRNA for Terminal A76-Addition by A-Adding Enzyme.
Yamashita S, Martinez A, Tomita K
Structure 23 830-842 (2015)
PMID: 25914059

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130371 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
A-adding tRNA nucleotidyltransferase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 46.55 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O66728 (Residues: 443-824; Coverage: 46%)
Gene name: aq_411
Sequence domains: Poly A polymerase head domain
Structure domains:
RNA (74-MER) Chain: B
Molecule details ›
Chain: B
Length: 74 nucleotides
Theoretical weight: 23.85 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21212
Unit cell:
a: 59.31Å b: 111.76Å c: 127.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.277 0.302
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

The tRNA Elbow in Structure, Recognition and Evolution.
Zhang et al. (2016)
3 more