› 4w4o

X-ray diffraction
1.8Å resolution

High-resolution crystal structure of Fc bound to its human receptor Fc-gamma-RI

Released:
DOI: 10.2210/pdb4w4o/pdb
PDB entry 4w4o coloured by chain, front view.
PDB entry 4w4o coloured by chain, side view.
PDB entry 4w4o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for binding of human IgG1 to its high-affinity human receptor FcγRI.
Kiyoshi M, Caaveiro JM, Kawai T, Tashiro S, Ide T, Asaoka Y, Hatayama K, Tsumoto K
Nat Commun 6 6866 (2015)
PMID: 25925696

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134855 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 25.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01857 (Residues: 107-328; Coverage: 56%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
High affinity immunoglobulin gamma Fc receptor I Chain: C
Molecule details ›
Chain: C
Length: 280 amino acids
Theoretical weight: 31.55 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P12314 (Residues: 16-289; Coverage: 76%)
Gene names: FCG1, FCGR1, FCGR1A, IGFR1
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand ZN 6 x ZN
Ligand ACT 9 x ACT
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 134.98Å b: 126.5Å c: 71.61Å
α: 90° β: 118.95° γ: 90°
R-values:
R R work R free
0.175 0.173 0.215
Expression systems:
  • Not provided
  • Spodoptera frugiperda

Quick links

Citations

26 review citations

Fcγ Receptor Function and the Design of Vaccination Strategies.
Bournazos et al. (2017)
25 more

15 mentions without citation

IgG Fc engineering to modulate antibody effector functions.
Wang et al. (2018)
14 more