› 4v81

X-ray diffraction
3.8Å resolution

The crystal structure of yeast CCT reveals intrinsic asymmetry of eukaryotic cytosolic chaperonins

Released:
DOI: 10.2210/pdb4v81/pdb
PDB entry 4v81 coloured by chain, front view.
PDB entry 4v81 coloured by chain, side view.
PDB entry 4v81 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of yeast CCT reveals intrinsic asymmetry of eukaryotic cytosolic chaperonins.
Dekker C, Roe SM, McCormack EA, Beuron F, Pearl LH, Willison KR
EMBO J 30 3078-90 (2011)
PMID: 21701561

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146270 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
T-complex protein 1 subunit alpha Chains: A, I, a, i
Molecule details ›
Chains: A, I, a, i
Length: 559 amino acids
Theoretical weight: 60.56 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P12612 (Residues: 1-559; Coverage: 100%)
Gene names: CCT1, TCP1, YD8142.13, YD8142B.04, YDR212W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit beta Chains: B, J, b, j
Molecule details ›
Chains: B, J, b, j
Length: 527 amino acids
Theoretical weight: 57.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39076 (Residues: 1-527; Coverage: 100%)
Gene names: BIN3, CCT2, TCP2, YIL142W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit gamma Chains: C, K, c, k
Molecule details ›
Chains: C, K, c, k
Length: 590 amino acids
Theoretical weight: 64.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39077 (Residues: 1-374, 375-534; Coverage: 100%)
Gene names: BIN2, CCT3, J1336, TCP3, YJL014W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit delta Chains: D, L, d, l
Molecule details ›
Chains: D, L, d, l
Length: 528 amino acids
Theoretical weight: 57.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39078 (Residues: 1-528; Coverage: 100%)
Gene names: ANC2, CCT4, TCP4, YDL143W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit epsilon Chains: E, M, e, m
Molecule details ›
Chains: E, M, e, m
Length: 562 amino acids
Theoretical weight: 62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40413 (Residues: 1-562; Coverage: 100%)
Gene names: CCT5, J1752, TCP5, YJR064W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit zeta Chains: F, N, f, n
Molecule details ›
Chains: F, N, f, n
Length: 546 amino acids
Theoretical weight: 60 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39079 (Residues: 1-546; Coverage: 100%)
Gene names: CCT6, TCP20, TCP6, YD9395.21, YDR188W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit eta Chains: G, O, g, o
Molecule details ›
Chains: G, O, g, o
Length: 550 amino acids
Theoretical weight: 59.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P42943 (Residues: 1-550; Coverage: 100%)
Gene names: CCT7, J0804, YJL111W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit theta Chains: H, P, h, p
Molecule details ›
Chains: H, P, h, p
Length: 568 amino acids
Theoretical weight: 61.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P47079 (Residues: 1-568; Coverage: 100%)
Gene names: CCT8, J1374, YJL008C
Sequence domains: TCP-1/cpn60 chaperonin family

Ligands and Environments

3 bound ligands:
Ligand ADP 24 x ADP
Ligand BEF 24 x BEF
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 159.1Å b: 162.54Å c: 268.1Å
α: 85.23° β: 81.15° γ: 61.17°
R-values:
R R work R free
0.309 0.307 0.344
Expression system: Saccharomyces cerevisiae

Quick links

Citations

17 review citations

Molecular chaperone functions in protein folding and proteostasis.
Kim et al. (2013)
16 more

4 mentions without citation

Structure of the human TRiC/CCT Subunit 5 associated with hereditary sensory neuropathy.
Pereira et al. (2017)
3 more