› 4uy0

Electron Microscopy
7.7Å resolution

Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins

Released:
DOI: 10.2210/pdb4uy0/pdb
PDB entry 4uy0 coloured by chain, front view.
PDB entry 4uy0 coloured by chain, side view.
PDB entry 4uy0 coloured by chain, top view.
Source organisms:
Primary publication:
Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins.
Atherton J, Farabella I, Yu IM, Rosenfeld SS, Houdusse A, Topf M, Moores CA
Elife 3 e03680 (2014)
PMID: 25209998
Related structures: EMD-2771

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-160589 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 426 amino acids
Theoretical weight: 47.81 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 2-427; Coverage: 96%)
Gene name: TUBB2B
Sequence domains:
Kinesin heavy chain isoform 5A Chain: C
Molecule details ›
Chain: C
Length: 340 amino acids
Theoretical weight: 38.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12840 (Residues: 1-340; Coverage: 33%)
Gene names: KIF5A, NKHC1
Sequence domains: Kinesin motor domain

Ligands and Environments

8 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 2 x MG
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
Ligand ADP 1 x ADP
Ligand ALF 1 x ALF
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.7Å
Relevant EMDB volumes: EMD-2771
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Axonal transport: Driving synaptic function.
Guedes-Dias et al. (2019)
16 more

3 mentions without citation

Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins.
Atherton et al. (2014)
2 more