› 4u90

X-ray diffraction
2Å resolution

GephE in complex with PEG crosslinked GABA receptor alpha3 subunit derived dimeric peptide

Released:
DOI: 10.2210/pdb4u90/pdb
PDB entry 4u90 coloured by chain, front view.
PDB entry 4u90 coloured by chain, side view.
PDB entry 4u90 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Design and synthesis of high-affinity dimeric inhibitors targeting the interactions between gephyrin and inhibitory neurotransmitter receptors.
Maric HM, Kasaragod VB, Haugaard-Kedström L, Hausrat TJ, Kneussel M, Schindelin H, Strømgaard K
Angew Chem Int Ed Engl 54 490-4 (2015)
PMID: 25413248

Function and Biology Details

Reactions catalysed: 
Adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP
ATP + molybdopterin = diphosphate + adenylyl-molybdopterin
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148881 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gephyrin Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 45.65 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03555 (Residues: 350-768; Coverage: 55%)
Gene names: Gph, Gphn
Sequence domains:
Structure domains:
Gamma-aminobutyric acid receptor subunit alpha-3 Chains: D, E
Molecule details ›
Chains: D, E
Length: 10 amino acids
Theoretical weight: 1.11 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P20236 (Residues: 396-404; Coverage: 2%)
Gene names: Gabra-3, Gabra3

Ligands and Environments

2 bound ligands:
Ligand BU1 1 x BU1
Ligand 3F8 1 x 3F8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I222
Unit cell:
a: 88.538Å b: 99.24Å c: 114.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.211
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Binding Revisited-Avidity in Cellular Function and Signaling.
Erlendsson et al. (2020)
8 other articles

3 mentions without citation

Structure-Function Relationships of Glycine and GABAA Receptors and Their Interplay With the Scaffolding Protein Gephyrin.
Kasaragod et al. (2018)
2 more