› 4u46

X-ray diffraction
1.95Å resolution

Crystal structure of an avidin mutant

Released:
DOI: 10.2210/pdb4u46/pdb
PDB entry 4u46 coloured by chain, front view.
PDB entry 4u46 coloured by chain, side view.
PDB entry 4u46 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Artificial Avidin-Based Receptors for a Panel of Small Molecules.
Lehtonen SI, Tullila A, Agrawal N, Kukkurainen S, Kähkönen N, Koskinen M, Nevanen TK, Johnson MS, Airenne TT, Kulomaa MS, Riihimäki TA, Hytönen VP
ACS Chem Biol 11 211-21 (2016)
PMID: 26550684

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 15.86 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02701 (Residues: 25-152; Coverage: 100%)
Gene name: AVD
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand CL 12 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 74.48Å b: 79.8Å c: 43.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Structural characterization of core-bradavidin in complex with biotin.
Agrawal et al. (2017)

1 mention without citation

Molecular features of steroid-binding antidins and their use for assaying serum progesterone.
Agrawal et al. (2019)