› 4u31

X-ray diffraction
1.85Å resolution

Sco GlgEI-V279S in Complex with maltose-C-phosphonate

Released:
DOI: 10.2210/pdb4u31/pdb
PDB entry 4u31 coloured by chain, front view.
PDB entry 4u31 coloured by chain, side view.
PDB entry 4u31 coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Crystal structures of Mycobacterium tuberculosis GlgE and complexes with non-covalent inhibitors.
Lindenberger JJ, Veleti SK, Wilson BN, Sucheck SJ, Ronning DR
Sci Rep 5 12830 (2015)
PMID: 26245983

Function and Biology Details

Reaction catalysed: 
Alpha-maltose 1-phosphate + ((1->4)-alpha-D-glucosyl)(n) = phosphate + ((1->4)-alpha-D-glucosyl)(n+2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 683 amino acids
Theoretical weight: 76.39 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L1K2 (Residues: 1-675; Coverage: 100%)
Gene names: SC6A11.19c, SCO5443, glgE1, pep1, pep1A, pep1I
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GPM, GLC
Ligand GPM 2 x GPM
Ligand GLC 2 x GLC
2 bound ligands:
Ligand CIT 2 x CIT
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41212
Unit cell:
a: 113.556Å b: 113.556Å c: 315.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.194
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Target-based approaches for the discovery of new antimycobacterial drugs.
Borsari et al. (2017)
2 more

5 mentions without citation

Synthesis of 2-deoxy-2,2-difluoro-α-maltosyl fluoride and its X-ray structure in complex with Streptomyces coelicolor GlgEI-V279S.
Thanna et al. (2015)
4 more