› 4typ

X-ray diffraction
2.9Å resolution

Crystal structure of an adenylate kinase mutant--AKm1

Released:
DOI: 10.2210/pdb4typ/pdb
PDB entry 4typ coloured by chain, front view.
PDB entry 4typ coloured by chain, side view.
PDB entry 4typ coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Crystal structures of thermally stable adenylate kinase mutants designed by local structural entropy optimization and structure-guided mutagenesis
Moon S, Bae E
J KOREAN SOC APPL BIOL CHEM 57 661-665 (2014)

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 217 amino acids
Theoretical weight: 24.46 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16304 (Residues: 1-217; Coverage: 100%)
Gene names: BSU01370, adk
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand AP5 4 x AP5
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 43.641Å b: 123.338Å c: 86.693Å
α: 90° β: 98.28° γ: 90°
R-values:
R R work R free
0.29 0.288 0.329
Expression system: Escherichia coli BL21(DE3)

Quick links