› 4twv

X-ray diffraction
1.06Å resolution

Horse heart myoglobin mutant (K45E/K63E/K96E) with Zn-deuteroporphyrin IX

Released:
DOI: 10.2210/pdb4twv/pdb
PDB entry 4twv coloured by chain, front view.
PDB entry 4twv coloured by chain, side view.
PDB entry 4twv coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Charge-Disproportionation Symmetry Breaking Creates a Heterodimeric Myoglobin Complex with Enhanced Affinity and Rapid Intracomplex Electron Transfer.
Trana EN, Nocek JM, Woude JV, Span I, Smith SM, Rosenzweig AC, Hoffman BM
J Am Chem Soc 138 12615-28 (2016)
PMID: 27646786

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.11 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68082 (Residues: 1-154; Coverage: 100%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand ZND 1 x ZND
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 28.53Å b: 34.97Å c: 123.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.165
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Design of artificial metalloproteins/metalloenzymes by tuning noncovalent interactions.
Hirota et al. (2018)
2 other articles

2 mentions without citation

Charge-Disproportionation Symmetry Breaking Creates a Heterodimeric Myoglobin Complex with Enhanced Affinity and Rapid Intracomplex Electron Transfer.
Trana et al. (2016)
1 more