› 4ttv

X-ray diffraction
2.8Å resolution

Crystal structure of human ThrRS complexing with a bioengineered macrolide BC194

Released:
DOI: 10.2210/pdb4ttv/pdb
PDB entry 4ttv coloured by chain, front view.
PDB entry 4ttv coloured by chain, side view.
PDB entry 4ttv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Aminoacyl-tRNA synthetase dependent angiogenesis revealed by a bioengineered macrolide inhibitor.
Mirando AC, Fang P, Williams TF, Baldor LC, Howe AK, Ebert AM, Wilkinson B, Lounsbury KM, Guo M, Francklyn CS
Sci Rep 5 13160 (2015)
PMID: 26271225

Function and Biology Details

Reaction catalysed: 
ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine--tRNA ligase 1, cytoplasmic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 414 amino acids
Theoretical weight: 48.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26639 (Residues: 322-723; Coverage: 56%)
Gene names: TARS, TARS1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BC9 4 x BC9
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 92.02Å b: 134.61Å c: 128.67Å
α: 90° β: 90.1° γ: 90°
R-values:
R R work R free
0.256 0.255 0.288
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Protein moonlighting: what is it, and why is it important?
Jeffery CJ. (2018)
5 more

4 mentions without citation

Aminoacyl-tRNA synthetase dependent angiogenesis revealed by a bioengineered macrolide inhibitor.
Mirando et al. (2015)
3 more