› 4ts1

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF A DELETION MUTANT OF A TYROSYL-T/RNA SYNTHETASE COMPLEXED WITH TYROSINE

Released:
DOI: 10.2210/pdb4ts1/pdb
PDB entry 4ts1 coloured by chain, front view.
PDB entry 4ts1 coloured by chain, side view.
PDB entry 4ts1 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure of a deletion mutant of a tyrosyl-tRNA synthetase complexed with tyrosine.
Brick P, Blow DM
J Mol Biol 194 287-97 (1987)
PMID: 3612807

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 36.36 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
UniProt:
  • Canonical: P00952 (Residues: 1-317; Coverage: 76%)
Gene name: tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TYR 2 x TYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 95.46Å b: 67.06Å c: 61.37Å
α: 90° β: 90.78° γ: 90°
R-values:
R R work R free
0.187 not available not available
Expression system: Not provided

Quick links

Citations

12 review citations

Aminoacyl-tRNA synthesis.
Ibba et al. (2000)
11 more

7 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
6 more