› 4tms

X-ray diffraction
2.35Å resolution

PLASTIC ADAPTATION TOWARD MUTATIONS IN PROTEINS: STRUCTURAL COMPARISON OF THYMIDYLATE SYNTHASES

Released:
DOI: 10.2210/pdb4tms/pdb
PDB entry 4tms coloured by chain, front view.
PDB entry 4tms coloured by chain, side view.
PDB entry 4tms coloured by chain, top view.
Source organism: Lacticaseibacillus casei
Primary publication:
Plastic adaptation toward mutations in proteins: structural comparison of thymidylate synthases.
Perry KM, Fauman EB, Finer-Moore JS, Montfort WR, Maley GF, Maley F, Stroud RM
Proteins 8 315-33 (1990)
PMID: 2128651

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132544 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 36.63 KDa
Source organism: Lacticaseibacillus casei
Expression system: Not provided
UniProt:
  • Canonical: P00469 (Residues: 1-316; Coverage: 100%)
Gene name: thyA
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P6122
Unit cell:
a: 78.3Å b: 78.3Å c: 243.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 not available not available
Expression system: Not provided

Quick links

Citations

7 review citations

Tetrahydrofolate and tetrahydromethanopterin compared: functionally distinct carriers in C1 metabolism.
Maden BE. (2000)
6 more

10 mentions without citation

Viral and cellular MARCH ubiquitin ligases and cancer.
Wang et al. (2008)
9 more