› 4s0h

X-ray diffraction
2.82Å resolution

TBX5 DB, NKX2.5 HD, ANF DNA Complex

Released:
DOI: 10.2210/pdb4s0h/pdb
PDB entry 4s0h coloured by chain, front view.
PDB entry 4s0h coloured by chain, side view.
PDB entry 4s0h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Intermolecular Interactions of Cardiac Transcription Factors NKX2.5 and TBX5.
Pradhan L, Gopal S, Li S, Ashur S, Suryanarayanan S, Kasahara H, Nam HJ
Biochemistry 55 1702-10 (2016)
PMID: 26926761

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118156 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
T-box transcription factor TBX5 Chains: A, E
Molecule details ›
Chains: A, E
Length: 186 amino acids
Theoretical weight: 21.4 KDa
Source organism: Homo sapiens
Expression system: unidentified
UniProt:
  • Canonical: Q99593 (Residues: 53-238; Coverage: 36%)
Gene name: TBX5
Sequence domains: T-box
Structure domains: Transcription factor, T-box
Homeobox protein Nkx-2.5 Chains: B, F
Molecule details ›
Chains: B, F
Length: 53 amino acids
Theoretical weight: 6.53 KDa
Source organism: Homo sapiens
Expression system: unidentified
UniProt:
  • Canonical: P52952 (Residues: 142-194; Coverage: 16%)
Gene names: CSX, NKX2-5, NKX2.5, NKX2E
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
5'-D(*TP*CP*TP*CP*AP*CP*AP*CP*CP*TP*TP*TP*GP*AP*AP*GP*TP*GP*G)-3' Chains: C, G
Molecule details ›
Chains: C, G
Length: 19 nucleotides
Theoretical weight: 5.8 KDa
5'-D(*CP*CP*AP*CP*TP*TP*CP*AP*AP*AP*GP*GP*TP*GP*TP*GP*AP*GP*A)-3' Chains: D, H
Molecule details ›
Chains: D, H
Length: 19 nucleotides
Theoretical weight: 5.85 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 70.217Å b: 78.459Å c: 78.695Å
α: 90° β: 108.83° γ: 90°
R-values:
R R work R free
0.195 0.189 0.248
Expression system: unidentified

Quick links

Citations

3 review citations

Targeting GATA4 for cardiac repair.
Välimäki et al. (2020)
2 more