› 4ryy

X-ray diffraction
2.3Å resolution

Crystal structure of RPE65 in complex with R-emixustat and palmitate

Released:
DOI: 10.2210/pdb4ryy/pdb
PDB entry 4ryy coloured by chain, front view.
PDB entry 4ryy coloured by chain, side view.
PDB entry 4ryy coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Molecular pharmacodynamics of emixustat in protection against retinal degeneration.
Zhang J, Kiser PD, Badiee M, Palczewska G, Dong Z, Golczak M, Tochtrop GP, Palczewski K
J Clin Invest 125 2781-94 (2015)
PMID: 26075817

Function and Biology Details

Reactions catalysed: 
An all-trans-retinyl ester + H(2)O = 11-cis-retinol + a fatty acid
Lutein = meso-zeaxanthin
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinoid isomerohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 533 amino acids
Theoretical weight: 60.94 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q28175 (Residues: 2-533; Coverage: 100%)
Gene name: RPE65
Sequence domains: Retinal pigment epithelial membrane protein

Ligands and Environments

3 bound ligands:
Ligand FE2 2 x FE2
Ligand PLM 2 x PLM
Ligand A3V 2 x A3V
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P65
Unit cell:
a: 175.844Å b: 175.844Å c: 86.424Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.215

Quick links

Citations

15 review citations

Retinoids and Retinal Diseases.
Kiser et al. (2016)
14 more

2 mentions without citation

Molecular pharmacodynamics of emixustat in protection against retinal degeneration.
Zhang et al. (2015)
1 more