› 4rsg

Neutron Diffraction
1.91Å resolution

Neutron crystal structure of Ras bound to the GTP analogue GppNHp

Released:
DOI: 10.2210/pdb4rsg/pdb
PDB entry 4rsg coloured by chain, front view.
PDB entry 4rsg coloured by chain, side view.
PDB entry 4rsg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Neutron Crystal Structure of RAS GTPase Puts in Question the Protonation State of the GTP γ-Phosphate.
Knihtila R, Holzapfel G, Weiss K, Meilleur F, Mattos C
J Biol Chem 290 31025-36 (2015)
PMID: 26515069

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
Ligand DOD 75 x DOD
No modified residues

Experiments and Validation Details

Spacegroup: P3221
Unit cell:
a: 40.084Å b: 40.084Å c: 160.785Å α: 90° β: 90° γ: 120°
R-values:
R R work R free 0.253 0.249 0.288
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The Ras Superfamily of Small GTPases in Non-neoplastic Cerebral Diseases.
Qu et al. (2019)
4 more

10 mentions without citation

The K-Ras, N-Ras, and H-Ras Isoforms: Unique Conformational Preferences and Implications for Targeting Oncogenic Mutants.
Parker et al. (2018)
9 more