4rgn

X-ray diffraction
2.7Å resolution

Structure of Staphylococcal Enterotoxin B bound to two neutralizing antibodies, 14G8 and 6D3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-208434 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Enterotoxin type B Chains: A, S
Molecule details ›
Chains: A, S
Length: 245 amino acids
Theoretical weight: 28.98 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01552 (Residues: 28-266; Coverage: 100%)
Gene name: entB
Sequence domains:
Structure domains:
14G8 heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 222 amino acids
Theoretical weight: 23.78 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
14G8 light chain Chains: C, L
Molecule details ›
Chains: C, L
Length: 214 amino acids
Theoretical weight: 23.59 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
6D3 heavy chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 221 amino acids
Theoretical weight: 23.65 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
6D3 light chain Chains: E, G
Molecule details ›
Chains: E, G
Length: 220 amino acids
Theoretical weight: 24.36 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 301.331Å b: 109.818Å c: 82.782Å
α: 90° β: 94.53° γ: 90°
R-values:
R R work R free
0.242 0.24 0.278
Expression system: Escherichia coli BL21(DE3)