› 4rfs

X-ray diffraction
3.23Å resolution

Structure of a pantothenate energy coupling factor transporter

Released:
DOI: 10.2210/pdb4rfs/pdb
PDB entry 4rfs coloured by chain, front view.
PDB entry 4rfs coloured by chain, side view.
PDB entry 4rfs coloured by chain, top view.
Source organism: Levilactobacillus brevis
Primary publication:
Structure of a pantothenate transporter and implications for ECF module sharing and energy coupling of group II ECF transporters.
Zhang M, Bao Z, Zhao Q, Guo H, Xu K, Wang C, Zhang P
Proc Natl Acad Sci U S A 111 18560-5 (2014)
PMID: 25512487

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-169973 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Energy-coupling factor transporter ATP-binding protein EcfA2 Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.18 KDa
Source organism: Levilactobacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03PY6 (Residues: 1-290; Coverage: 100%)
Gene names: LVIS_1661, cbiO2, ecfA2
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Energy-coupling factor transporter ATP-binding protein EcfA1 Chain: B
Molecule details ›
Chain: B
Length: 279 amino acids
Theoretical weight: 30.55 KDa
Source organism: Levilactobacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03PY5 (Residues: 1-279; Coverage: 100%)
Gene names: LVIS_1662, cbiO1, ecfA1
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
ECF transporter S component Chain: S
Molecule details ›
Chain: S
Length: 203 amino acids
Theoretical weight: 22.21 KDa
Source organism: Levilactobacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03SM0 (Residues: 1-203; Coverage: 100%)
Gene name: LVIS_0658
Sequence domains: ECF transporter, substrate-specific component
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3
Energy-coupling factor transporter transmembrane protein EcfT Chain: T
Molecule details ›
Chain: T
Length: 280 amino acids
Theoretical weight: 31.94 KDa
Source organism: Levilactobacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03PY7 (Residues: 1-266; Coverage: 100%)
Gene names: LVIS_1660, ecfT
Sequence domains: Cobalt transport protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 77.726Å b: 145.24Å c: 157.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.289
Expression system: Escherichia coli

Quick links

Citations

1 review citation

ECF-Type ATP-Binding Cassette Transporters.
Rempel et al. (2019)
18 other articles

9 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
8 more