› 4qrv

X-ray diffraction
1.98Å resolution

Crystal structure of I86F mutant of papain

Released:
DOI: 10.2210/pdb4qrv/pdb
PDB entry 4qrv coloured by chain, front view.
PDB entry 4qrv coloured by chain, side view.
PDB entry 4qrv coloured by chain, top view.
Source organism: Carica papaya
Entry authors: Dutta S, Choudhury D, Roy S

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Papain Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 41.1 KDa
Source organism: Carica papaya
Expression system: Escherichia coli
UniProt:
  • Canonical: P00784 (Residues: 27-345; Coverage: 98%)
Sequence domains:
Structure domains: Cysteine proteinases

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 42.67Å b: 74.267Å c: 116.326Å
α: 90° β: 92.85° γ: 90°
R-values:
R R work R free
0.201 0.198 0.249
Expression system: Escherichia coli

Quick links