› 4qnr

X-ray diffraction
1.54Å resolution

CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP

Released:
DOI: 10.2210/pdb4qnr/pdb
PDB entry 4qnr coloured by chain, front view.
PDB entry 4qnr coloured by chain, side view.
PDB entry 4qnr coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular basis of nucleotide-dependent substrate engagement and remodeling by an AAA+ activator.
Darbari VC, Lawton E, Lu D, Burrows PC, Wiesler S, Joly N, Zhang N, Zhang X, Buck M
Nucleic Acids Res 42 9249-61 (2014)
PMID: 25063294

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Psp operon transcriptional activator Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37344 (Residues: 1-265; Coverage: 82%)
Gene names: JW1296, b1303, pspF, ycjB
Sequence domains: Sigma-54 interaction domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 1 x MG
Ligand GOL 2 x GOL
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P65
Unit cell:
a: 113.555Å b: 113.555Å c: 39.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.147 0.178
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Neuromodulators as Interdomain Signaling Molecules Capable of Occupying Effector Binding Sites in Bacterial Transcription Factors.
Purtov et al. (2023)
1 more