› 4q2v

X-ray diffraction
2.2Å resolution

Crystal Structure of Ricin A chain complexed with Baicalin inhibitor

Released:
DOI: 10.2210/pdb4q2v/pdb
PDB entry 4q2v coloured by chain, front view.
PDB entry 4q2v coloured by chain, side view.
PDB entry 4q2v coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
Baicalin inhibits the lethality of ricin in mice by inducing protein oligomerization.
Dong J, Zhang Y, Chen Y, Niu X, Zhang Y, Li R, Yang C, Wang Q, Li X, Deng X
J Biol Chem 290 12899-907 (2015)
PMID: 25847243

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 31.26 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02879 (Residues: 36-302; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 0XE 1 x 0XE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 68.197Å b: 68.197Å c: 133.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.243
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Treatments for Pulmonary Ricin Intoxication: Current Aspects and Future Prospects.
Gal et al. (2017)
2 more

2 mentions without citation

Hosts and Heterologous Expression Strategies of Recombinant Toxins for Therapeutic Purposes.
di Leandro et al. (2023)
1 more