› 4pxg

X-ray diffraction
2.45Å resolution

Crystal Structure of TypeII restriction Enzyme Sau3AI

Released:
DOI: 10.2210/pdb4pxg/pdb
PDB entry 4pxg coloured by chain, front view.
PDB entry 4pxg coloured by chain, side view.
PDB entry 4pxg coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry authors: Xu CY, Yu F, Hu XJ, He JH

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme Sau3AI Chains: A, B
Molecule details ›
Chains: A, B
Length: 509 amino acids
Theoretical weight: 59.09 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P16667 (Residues: 1-489; Coverage: 100%)
Gene name: sau3AIR
Sequence domains: DNA mismatch repair enzyme MutH

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A, SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 69.648Å b: 197.673Å c: 191.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.263
Expression system: Escherichia coli

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