› 4pfh

X-ray diffraction
1.9Å resolution

Crystal structure of engineered D-tagatose 3-epimerase PcDTE-IDF8

Released:
DOI: 10.2210/pdb4pfh/pdb
PDB entry 4pfh coloured by chain, front view.
PDB entry 4pfh coloured by chain, side view.
PDB entry 4pfh coloured by chain, top view.
Source organism: Pseudomonas cichorii
Primary publication:
Directed divergent evolution of a thermostable D-tagatose epimerase towards improved activity for two hexose substrates.
Bosshart A, Hee CS, Bechtold M, Schirmer T, Panke S
Chembiochem 16 592-601 (2015)
PMID: 25655925

Function and Biology Details

Reaction catalysed: 
D-psicose = D-fructose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-tagatose 3-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 33.92 KDa
Source organism: Pseudomonas cichorii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O50580 (Residues: 1-290; Coverage: 100%)
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

3 bound ligands:
Ligand MN 3 x MN
Ligand PSJ 2 x PSJ
Ligand FUD 2 x FUD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 57.46Å b: 86.74Å c: 61.82Å
α: 90° β: 99.1° γ: 90°
R-values:
R R work R free
0.146 0.144 0.177
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Enzymatic approaches to rare sugar production.
Zhang et al. (2017)
1 more

3 mentions without citation

Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes.
Xia et al. (2021)
2 more