› 4pcu

X-ray diffraction
3.58Å resolution

Crystal structure of delta516-525 E201S human cystathionine beta-synthase with AdoMet

Released:
DOI: 10.2210/pdb4pcu/pdb
PDB entry 4pcu coloured by chain, front view.
PDB entry 4pcu coloured by chain, side view.
PDB entry 4pcu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into the molecular mechanism of allosteric activation of human cystathionine β-synthase by S-adenosylmethionine.
Ereño-Orbea J, Majtan T, Oyenarte I, Kraus JP, Martínez-Cruz LA
Proc Natl Acad Sci U S A 111 E3845-52 (2014)
PMID: 25197074

Function and Biology Details

Reaction catalysed: 
L-serine + L-homocysteine = L-cystathionine + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 60.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35520 (Residues: 1-551; Coverage: 98%)
Gene name: CBS
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP

Cofactor: Ligand SAM 2 x SAM

Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P3121
Unit cell:
a: 141.354Å b: 141.354Å c: 108.528Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.257 0.277
Expression system: Escherichia coli

Quick links

Citations

15 review citations

H2S: A Novel Gasotransmitter that Signals by Sulfhydration.
Paul et al. (2015)
14 more

12 mentions without citation

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
11 more