› 4pa0

X-ray diffraction
2.25Å resolution

Omecamtiv Mercarbil binding site on the Human Beta-Cardiac Myosin Motor Domain

Released:
DOI: 10.2210/pdb4pa0/pdb
PDB entry 4pa0 coloured by chain, front view.
PDB entry 4pa0 coloured by chain, side view.
PDB entry 4pa0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for drug-induced allosteric changes to human β-cardiac myosin motor activity.
Winkelmann DA, Forgacs E, Miller MT, Stock AM
Nat Commun 6 7974 (2015)
PMID: 26246073

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146361 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Myosin-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1024 amino acids
Theoretical weight: 116.61 KDa
Source organisms: Expression system: Mus
UniProt:
  • Canonical: P12883 (Residues: 1-787; Coverage: 41%)
  • Canonical: P42212 (Residues: 5-234; Coverage: 97%)
Gene names: GFP, MYH7, MYHCB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand 2OW 2 x 2OW
Ligand GOL 3 x GOL
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 100.167Å b: 88.924Å c: 137.786Å
α: 90° β: 93.92° γ: 90°
R-values:
R R work R free
0.203 0.201 0.246
Expression system: Mus

Quick links

Citations

11 review citations

Hypertrophic cardiomyopathy and the myosin mesa: viewing an old disease in a new light.
Trivedi et al. (2018)
10 more

14 mentions without citation

Cardiac myosin contraction and mechanotransduction in health and disease.
Barrick et al. (2021)
13 more