› 4p5s

X-ray diffraction
1.02Å resolution

Structure of reduced W45Y mutant of amicyanin

Released:
DOI: 10.2210/pdb4p5s/pdb
PDB entry 4p5s coloured by chain, front view.
PDB entry 4p5s coloured by chain, side view.
PDB entry 4p5s coloured by chain, top view.
Source organism: Paracoccus denitrificans
Primary publication:
The sole tryptophan of amicyanin enhances its thermal stability but does not influence the electronic properties of the type 1 copper site.
Dow BA, Sukumar N, Matos JO, Choi M, Schulte A, Tatulian SA, Davidson VL
Arch Biochem Biophys 550-551 20-7 (2014)
PMID: 24704124

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amicyanin Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.48 KDa
Source organism: Paracoccus denitrificans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22364 (Residues: 27-131; Coverage: 100%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU1 1 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 27.165Å b: 56.646Å c: 28.663Å
α: 90° β: 96.67° γ: 90°
R-values:
R R work R free
0.135 0.134 0.15
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Intrinsic tryptophan fluorescence in the detection and analysis of proteins: a focus on Förster resonance energy transfer techniques.
Ghisaidoobe et al. (2014)
1 more

2 mentions without citation

The sole tryptophan of amicyanin enhances its thermal stability but does not influence the electronic properties of the type 1 copper site.
Dow et al. (2014)
1 more