› 4p2v

X-ray diffraction
2.51Å resolution

Structure of the AI-2 processing enzyme LsrF in complex with the product of the LsrG reaction P-HPD

Released:
DOI: 10.2210/pdb4p2v/pdb
PDB entry 4p2v coloured by chain, front view.
PDB entry 4p2v coloured by chain, side view.
PDB entry 4p2v coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2.
Marques JC, Oh IK, Ly DC, Lamosa P, Ventura MR, Miller ST, Xavier KB
Proc Natl Acad Sci U S A 111 14235-40 (2014)
PMID: 25225400

Function and Biology Details

Reaction catalysed: 
Glycerone phosphate + acetyl-CoA = 3-hydroxy-2,4-dioxopentyl phosphate + CoA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160059 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase Chains: A, B, C, D, E, F, G, H, I, K
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, K
Length: 293 amino acids
Theoretical weight: 32.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P76143 (Residues: 1-291; Coverage: 100%)
Gene names: JW1510, b1517, lsrF, yneB
Sequence domains: DeoC/LacD family aldolase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand 26T 10 x 26T
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 78.617Å b: 105.742Å c: 170.402Å
α: 90° β: 101.43° γ: 90°
R-values:
R R work R free
0.237 0.235 0.271
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Acetate metabolism regulation in Escherichia coli: carbon overflow, pathogenicity, and beyond.
Bernal et al. (2016)
2 more

2 mentions without citation

LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2.
Marques et al. (2014)
1 more