› 4p2o

X-ray diffraction
2.6Å resolution

Crystal structure of the 2B4 TCR in complex with 2A/I-Ek

Released:
DOI: 10.2210/pdb4p2o/pdb
PDB entry 4p2o coloured by chain, front view.
PDB entry 4p2o coloured by chain, side view.
PDB entry 4p2o coloured by chain, top view.
Source organisms:
Primary publication:
Deconstructing the peptide-MHC specificity of T cell recognition.
Birnbaum ME, Mendoza JL, Sethi DK, Dong S, Glanville J, Dobbins J, Ozkan E, Davis MM, Wucherpfennig KW, Garcia KC
Cell 157 1073-87 (2014)
PMID: 24855945

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-208995 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
H-2 class II histocompatibility antigen, E-K alpha chain Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04224 (Residues: 26-216; Coverage: 83%)
Sequence domains:
Structure domains:
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 236 amino acids
Theoretical weight: 26.75 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q31163 (Residues: 29-224; Coverage: 82%)
Gene name: H2-Eb1
Sequence domains:
Structure domains:
2B4 T-cell receptor alpha chain Chain: C
Molecule details ›
Chain: C
Length: 220 amino acids
Theoretical weight: 24.1 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
2B4 T-cell receptor beta chain Chain: D
Molecule details ›
Chain: D
Length: 255 amino acids
Theoretical weight: 29.01 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
2A peptide Chain: P
Molecule details ›
Chain: P
Length: 22 amino acids
Theoretical weight: 2.08 KDa
Source organism: synthetic construct
Expression system: Trichoplusia ni

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 239.936Å b: 60.176Å c: 78.359Å
α: 90° β: 104.33° γ: 90°
R-values:
R R work R free
0.192 0.189 0.245
Expression system: Trichoplusia ni

Quick links

Citations

112 review citations

The evolving landscape of biomarkers for checkpoint inhibitor immunotherapy.
Havel et al. (2019)
111 more

2 mentions without citation

Deconstructing the peptide-MHC specificity of T cell recognition.
Birnbaum et al. (2014)
1 more