› 4p1c

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE TOLUENE 4-MONOOXYGENASE HYDROXYLASE-FERREDOXIN C7S, C84A, C85A VARIANT ELECTRON-TRANSFER COMPLEX

Released:
DOI: 10.2210/pdb4p1c/pdb
PDB entry 4p1c coloured by chain, front view.
PDB entry 4p1c coloured by chain, side view.
PDB entry 4p1c coloured by chain, top view.
Source organism: Pseudomonas mendocina
Primary publication:
Structural basis for biomolecular recognition in overlapping binding sites in a diiron enzyme system.
Acheson JF, Bailey LJ, Elsen NL, Fox BG
Nat Commun 5 5009 (2014)
PMID: 25248368

Function and Biology Details

Reaction catalysed: 
Toluene + NADH + O(2) = 4-methylphenol + NAD(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169418 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Toluene-4-monooxygenase system, hydroxylase component subunit alpha Chains: A, D
Molecule details ›
Chains: A, D
Length: 490 amino acids
Theoretical weight: 57.09 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00456 (Residues: 2-491; Coverage: 98%)
Gene name: tmoA
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Structure domains: Ribonucleotide Reductase, subunit A
Toluene-4-monooxygenase system, hydroxylase component subunit beta Chains: B, E
Molecule details ›
Chains: B, E
Length: 305 amino acids
Theoretical weight: 35.93 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00460 (Residues: 2-306; Coverage: 93%)
Gene name: tmoE
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Structure domains: Ribonucleotide Reductase, subunit A
Toluene-4-monooxygenase system, hydroxylase component subunit gamma Chains: C, F
Molecule details ›
Chains: C, F
Length: 82 amino acids
Theoretical weight: 9.34 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00457 (Residues: 2-83; Coverage: 98%)
Gene name: tmoB
Sequence domains: Toluene-4-monooxygenase system protein B (TmoB)
Structure domains: TmoB-like
Toluene-4-monooxygenase system, ferredoxin component Chains: H, I
Molecule details ›
Chains: H, I
Length: 111 amino acids
Theoretical weight: 12.13 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00458 (Residues: 2-112; Coverage: 99%)
Gene name: tmoC
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

3 bound ligands:
Ligand FE 4 x FE
Ligand PEG 2 x PEG
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 95.227Å b: 106.353Å c: 213.418Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.153 0.215
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

A tale of two methane monooxygenases.
Ross et al. (2017)
2 more

1 mention without citation

Structural basis for biomolecular recognition in overlapping binding sites in a diiron enzyme system.
Acheson et al. (2014)