› 4p0e

X-ray diffraction
2.3Å resolution

YhdE E33A (p212121 space group)

Released:
DOI: 10.2210/pdb4p0e/pdb
PDB entry 4p0e coloured by chain, front view.
PDB entry 4p0e coloured by chain, side view.
PDB entry 4p0e coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Jia Z, Zheng J, Jin J, Wang N

Function and Biology Details

Reaction catalysed: 
A nucleoside triphosphate + H(2)O = a nucleotide + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dTTP/UTP pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 20.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P25536 (Residues: 2-190; Coverage: 96%)
Gene names: JW3217, b3248, yhdE
Sequence domains: Maf-like protein
Structure domains: Maf protein

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 45.883Å b: 85.897Å c: 88.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.24
Expression system: Escherichia coli

Quick links

Citations

3 mentions without citation

Insights into the cellular function of YhdE, a nucleotide pyrophosphatase from Escherichia coli.
Jin et al. (2015)
2 more