› 4oz9

X-ray diffraction
2.24Å resolution

Crystal structure of MltF from Pseudomonas aeruginosa complexed with isoleucine

Released:
DOI: 10.2210/pdb4oz9/pdb
PDB entry 4oz9 coloured by chain, front view.
PDB entry 4oz9 coloured by chain, side view.
PDB entry 4oz9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Reddem E, Thunnissen AMWH

Function and Biology Details

Reaction catalysed: 
Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-bound lytic murein transglycosylase F Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 48.65 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HXN1 (Residues: 33-460; Coverage: 93%)
Gene names: PA3764, mltF
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand ILE 1 x ILE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 54.39Å b: 83.36Å c: 90.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.213 0.294
Expression system: Escherichia coli BL21(DE3)

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Citations

2 mentions without citation

Crystal Structures of the SpoIID Lytic Transglycosylases Essential for Bacterial Sporulation.
Nocadello et al. (2016)
1 more