› 4our

X-ray diffraction
3.4Å resolution

Crystal structure of Arabidopsis thaliana phytochrome B photosensory module

Released:
DOI: 10.2210/pdb4our/pdb
PDB entry 4our coloured by chain, front view.
PDB entry 4our coloured by chain, side view.
PDB entry 4our coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of the photosensing module from a red/far-red light-absorbing plant phytochrome.
Burgie ES, Bussell AN, Walker JM, Dubiel K, Vierstra RD
Proc Natl Acad Sci U S A 111 10179-84 (2014)
PMID: 24982198

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147033 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phytochrome B Chain: A
Molecule details ›
Chain: A
Length: 544 amino acids
Theoretical weight: 60.35 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P14713 (Residues: 90-624; Coverage: 46%)
Gene names: At2g18790, HY3, MSF3.17, OOP1, PHYB
Sequence domains:
Phytochrome B Chain: B
Molecule details ›
Chain: B
Length: 544 amino acids
Theoretical weight: 59.92 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P14713 (Residues: 90-624; Coverage: 46%)
Gene names: At2g18790, HY3, MSF3.17, OOP1, PHYB
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 11 x SO4
Ligand GOL 2 x GOL
Ligand O6E 2 x O6E
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41212
Unit cell:
a: 127.502Å b: 127.502Å c: 300.772Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.27
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Illuminating cell signalling with optogenetic tools.
Tischer et al. (2014)
28 more

23 mentions without citation

Optogenetic Immunomodulation: Shedding Light on Antitumor Immunity.
Tan et al. (2017)
22 more