› 4ooy

X-ray diffraction
1.1Å resolution

Avibactam and class C beta-lactamases: mechanism of inhibition, conservation of binding pocket and implications for resistance

Released:
DOI: 10.2210/pdb4ooy/pdb
PDB entry 4ooy coloured by chain, front view.
PDB entry 4ooy coloured by chain, side view.
PDB entry 4ooy coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Avibactam and class C β-lactamases: mechanism of inhibition, conservation of the binding pocket, and implications for resistance.
Lahiri SD, Johnstone MR, Ross PL, McLaughlin RE, Olivier NB, Alm RA
Antimicrob Agents Chemother 58 5704-13 (2014)
PMID: 25022578

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 359 amino acids
Theoretical weight: 39.37 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P24735 (Residues: 29-387; Coverage: 97%)
Gene names: PA4110, ampC
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand NXL 1 x NXL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 44.757Å b: 71.269Å c: 106.129Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.187
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Targeting Antibiotic Resistance.
Chellat et al. (2016)
29 more

4 mentions without citation

Structural Basis of Reduced Susceptibility to Ceftazidime-Avibactam and Cefiderocol in Enterobacter cloacae Due to AmpC R2 Loop Deletion.
Kawai et al. (2020)
3 more