› 4ooc

X-ray diffraction
2.7Å resolution

Dehydratase domain of the polyketide PpsC from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4ooc/pdb
PDB entry 4ooc coloured by chain, front view.
PDB entry 4ooc coloured by chain, side view.
PDB entry 4ooc coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Faille A, Mourey L, Pedelacq JD

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + 17-(4-hydroxyphenyl)heptadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH = C(35)-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP(+) + 5 CO(2) + 2 H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161211 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenolphthiocerol/phthiocerol polyketide synthase subunit C Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 34.46 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96202 (Residues: 921-1222; Coverage: 14%)
Gene names: Rv2933, ppsC
Sequence domains:
Structure domains: Polyketide synthase dehydratase

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29, ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 89.506Å b: 89.506Å c: 159.276Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.251
Expression system: Escherichia coli BL21(DE3)

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