› 4oiy

X-ray diffraction
1.5Å resolution

Crystal structure of Sec7p catalytic domain

Released:
DOI: 10.2210/pdb4oiy/pdb
PDB entry 4oiy coloured by chain, front view.
PDB entry 4oiy coloured by chain, side view.
PDB entry 4oiy coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
C-terminal motif within Sec7 domain regulates guanine nucleotide exchange activity via tuning protein conformation.
Qiu B, Zhang K, Wang S, Sun F
Biochem Biophys Res Commun 446 380-6 (2014)
PMID: 24613384

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein transport protein SEC7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.41 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P11075 (Residues: 824-1010; Coverage: 9%)
Gene names: SEC7, YD9395.01C, YD9489.05C, YDR170C
Sequence domains: Sec7 domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 48.647Å b: 68.961Å c: 59.605Å
α: 90° β: 106.74° γ: 90°
R-values:
R R work R free
0.212 0.211 0.237
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

The HUS box is required for allosteric regulation of the Sec7 Arf-GEF.
Halaby et al. (2018)