› 4oav

X-ray diffraction
2.1Å resolution

Complete human RNase L in complex with 2-5A (5'-ppp heptamer), AMPPCP and RNA substrate.

Released:
DOI: 10.2210/pdb4oav/pdb
PDB entry 4oav coloured by chain, front view.
PDB entry 4oav coloured by chain, side view.
PDB entry 4oav coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human RNase L reveals the basis for regulated RNA decay in the IFN response.
Han Y, Donovan J, Rath S, Whitney G, Chitrakar A, Korennykh A
Science 343 1244-8 (2014)
PMID: 24578532

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170256 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
2-5A-dependent ribonuclease Chains: B, D
Molecule details ›
Chains: B, D
Length: 699 amino acids
Theoretical weight: 79.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05823 (Residues: 21-719; Coverage: 94%)
Gene names: RNASEL, RNS4
Sequence domains:
Structure domains:
RNA (5'-R(P*(PO4)P*(PO4)P*AP*AP*AP*AP*(PO4))-2') Chains: A, C
Molecule details ›
Chains: A, C
Length: 7 nucleotides
Theoretical weight: 1.5 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ACP 2 x ACP
Ligand MG 4 x MG
Ligand PUP 1 x PUP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 58.61Å b: 160.7Å c: 230.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.229
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

28 review citations

The expanding regulatory mechanisms and cellular functions of circular RNAs.
Chen LL. (2020)
27 more

9 mentions without citation

Inhibition of the OAS/RNase L pathway by viruses.
Drappier et al. (2015)
8 more