› 4o36

X-ray diffraction
1.22Å resolution

Semisynthetic RNase S1-15-H7/11-Q10

Released:
DOI: 10.2210/pdb4o36/pdb
PDB entry 4o36 coloured by chain, front view.
PDB entry 4o36 coloured by chain, side view.
PDB entry 4o36 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
An Artificial Imine Reductase based on the Ribonuclease S scaffold
Genz M, Koehler V, Krauss M, Singer D, Hoffmann R, Ward TR, Strater N
ChemCatChem (2014)

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158391 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 15 amino acids
Theoretical weight: 1.74 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-41; Coverage: 12%)
Gene names: RNASE1, RNS1
Ribonuclease pancreatic Chain: B
Molecule details ›
Chain: B
Length: 104 amino acids
Theoretical weight: 11.56 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 47-150; Coverage: 84%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 43.91Å b: 43.91Å c: 96.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.184 0.226
Expression system: Not provided

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