› 4nx7

X-ray diffraction
1.15Å resolution

single cryogenic temperature model of DHFR

Released:
DOI: 10.2210/pdb4nx7/pdb
PDB entry 4nx7 coloured by chain, front view.
PDB entry 4nx7 coloured by chain, side view.
PDB entry 4nx7 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Integrated description of protein dynamics from room-temperature X-ray crystallography and NMR.
Fenwick RB, van den Bedem H, Fraser JS, Wright PE
Proc Natl Acad Sci U S A 111 E445-54 (2014)
PMID: 24474795

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABQ4 (Residues: 1-159; Coverage: 100%)
Gene names: JW0047, b0048, folA, tmrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
3 bound ligands:
Ligand MN 1 x MN
Ligand FOL 1 x FOL
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 34.04Å b: 44.82Å c: 98.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.139 0.169
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Protein Ensembles: How Does Nature Harness Thermodynamic Fluctuations for Life? The Diverse Functional Roles of Conformational Ensembles in the Cell.
Wei et al. (2016)
18 more

3 mentions without citation

Integrated description of protein dynamics from room-temperature X-ray crystallography and NMR.
Fenwick et al. (2014)
2 more