› 4nk7

X-ray diffraction
3.23Å resolution

Crystal Structure of the D. melanogaster Plk4 cryptic polo box (CPB)

Released:
DOI: 10.2210/pdb4nk7/pdb
PDB entry 4nk7 coloured by chain, front view.
PDB entry 4nk7 coloured by chain, side view.
PDB entry 4nk7 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of the C. elegans ZYG-1 cryptic polo box suggests a conserved mechanism for centriolar docking of Plk4 kinases.
Shimanovskaya E, Viscardi V, Lesigang J, Lettman MM, Qiao R, Svergun DI, Round A, Oegema K, Dong G
Structure 22 1090-1104 (2014)
PMID: 24980795

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-132174 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK4 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.5 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O97143 (Residues: 383-601; Coverage: 29%)
Gene names: CG7186, SAK
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6422
Unit cell:
a: 132.207Å b: 132.207Å c: 134.247Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.24 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Centrosome function and assembly in animal cells.
Conduit et al. (2015)
5 more

2 mentions without citation

Building a ninefold symmetrical barrel: structural dissections of centriole assembly.
Dong G. (2015)
1 more