› 4n07

X-ray diffraction
1.87Å resolution

Crystal structure of the GluA2 ligand-binding domain (S1S2J-L483Y-N754S) in complex with glutamate and BPAM-344 at 1.87 A resolution

Released:
DOI: 10.2210/pdb4n07/pdb
PDB entry 4n07 coloured by chain, front view.
PDB entry 4n07 coloured by chain, side view.
PDB entry 4n07 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Synthesis, pharmacological and structural characterization, and thermodynamic aspects of GluA2-positive allosteric modulators with a 3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide scaffold.
Nørholm AB, Francotte P, Olsen L, Krintel C, Frydenvang K, Goffin E, Challal S, Danober L, Botez-Pop I, Lestage P, Pirotte B, Kastrup JS
J Med Chem 56 8736-45 (2013)
PMID: 24131202

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 263 amino acids
Theoretical weight: 29.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-796; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

6 bound ligands:
Ligand 2J9 3 x 2J9
Ligand GLU 3 x GLU
Ligand ACT 13 x ACT
Ligand ZN 10 x ZN
Ligand CAC 1 x CAC
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21212
Unit cell:
a: 113.904Å b: 164.034Å c: 47.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.211
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.
Hansen et al. (2021)
3 more

1 mention without citation

Enthalpy-Entropy Compensation in the Binding of Modulators at Ionotropic Glutamate Receptor GluA2.
Krintel et al. (2016)