› 4mwh

X-ray diffraction
2.09Å resolution

Crystal structure of scCK2 alpha in complex with ATP

Released:
DOI: 10.2210/pdb4mwh/pdb
PDB entry 4mwh coloured by chain, front view.
PDB entry 4mwh coloured by chain, side view.
PDB entry 4mwh coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The multiple nucleotide-divalent cation binding modes of Saccharomyces cerevisiae CK2α indicate a possible co-substrate hydrolysis product (ADP/GDP) release pathway.
Liu H, Wang H, Teng M, Li X
Acta Crystallogr D Biol Crystallogr 70 501-13 (2014)
PMID: 24531484

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-147482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 44.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P15790 (Residues: 1-372; Coverage: 100%)
Gene names: CKA1, YIL035C
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 2 x MG
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 69.773Å b: 69.773Å c: 168.514Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.241
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Emerging roles of the αC-β4 loop in protein kinase structure, function, evolution, and disease.
Yeung et al. (2020)
1 other article

1 mention without citation

Structural analysis of fungal pathogenicity-related casein kinase α subunit, Cka1, in the human fungal pathogen Cryptococcus neoformans.
Ong et al. (2019)