› 4mi0

X-ray diffraction
2Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase EZH2 Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15910 (Residues: 520-746; Coverage: 30%)
Gene names: EZH2, KMT6
Sequence domains:
Structure domains: SET domain

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand UNX 4 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 45.01Å b: 57.796Å c: 74.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.239
Expression system: Spodoptera frugiperda

Quick links

Citations

26 review citations

HOTAIR: an oncogenic long non-coding RNA in different cancers.
Hajjari et al. (2015)
25 more

14 mentions without citation

Role of the Polycomb Repressive Complex 2 (PRC2) in Transcriptional Regulation and Cancer.
Laugesen et al. (2016)
13 more